The number of vegetarians and people following the vegan lifestyle increases from year to year. This may be due to ethical, religious, cultural or health reasons. How do you ensure that your vegan-declared food has really been processed without meat or animal derived products?
The Vegan Control™ kit, a qPCR based system for the detection of animal traces in food, is a quick and inexpensive solution to this Problem.
Type of PCR
TaqMan® assay based on FAM™ and HEX™ labeled probes.
Recommended Use / Scope
Can be used in research and industry for detection of meat/animal based contaminations of vegan foods. For research use only! Do not use for clinical diagnostic or for testing of patient samples.
Result Evaluation
Cycler based, real-time PCR
Sensitivity
Down to 0.1 % of genomic meat target DNA in a vegan food type background.
Content
Primer sets, nucleotides, internal amplification control and polymerase at optimized concentrations lyophilized in one tube, rehydration buffer, lyophilized positive control, PCR grade water.
Sample Requirements
Isolated total DNA from potentially contaminated vegan food serves as starting material. DNA can be extracted from sample material using DNA extraktion kits e.g. ExtractNow™ Vegan Control (Cat. No. 607-1050) or user method.
Required Consumables
1.5 ml reaction tubes, PCR reaction tubes, sterile filter tips
Required Lab Devices
qPCR cycler with FAM™ and HEX™ filter, variable microliter pipettes, benchtop centrifuge
Time to result
Approx. 150 minutes
Shelf Life and Storage
Components can be stored at +2 to +8 °C for at least 12 months. After rehydratisation the reagents must be stored at -18 °C.