The innuSPEED Stool DNA Kit provides an effective means of extracting microbial DNA from stool samples. The first step in the process is homogenization (e.g. with a SpeedMill) of the solid or liquid samples in a Lysis Tube using rapidly accelerated beads. This step is then followed by thermal lysis. This highly efficient homogenization process is followed by a prefiltration step, which serves as an optimum method for removing undissolved sample components. Microbial DNA are then selectively bound to the surface of a Spin Filter membrane; this is followed first by wash steps to remove inhibitors, and then by addition of a low-salt buffer to separate bound DNA from the membrane. The DNA is then ready for use in all subsequent applications.
Features
- Isolation of bacterial DNA from solid or liquid stool samples
- Includes prefiltration for removing undissolved sample components
- Lysis Tubes and adapted beads for optimum sample homogenization
- Suitable for use with homogenizers (such as the SpeedMill or others)