High quality single and multiple site-directed mutagenesis in less than 3 hours.
For single site mutagenesis, one kit accommodates both short and long templates (4-14 kb).
The kit delivers 80% mutagenesis efficiency for 1 site and 55% mutagenesis efficiency for 3 mutations simultaneously
The QuikChange Lightning Live Webinar is also available, helping analysts to accelerate research without sacrificing mutation efficiency, reliability or accuracy.
The kits use a rapid linear amplification method in which mutation-containing primers are incorporated but not copied.
Rapidly introduce point mutations, insertions, or deletions.
Agilent’s QuikChange Lightning kits offer a fast protocol for performing single and multiple site-directed mutagenesis. The kits utilize a rapid linear amplification method in which mutation-containing primers are incorporated but not copied. This enables effective mutagenesis to be performed in under 3 hours.
Other commercially available kits rely on PCR-based methods which may result in unwanted second-site errors. In addition, QuikChange Lightning site directed mutagenesis kits allow you to introduce point mutations, insertions, or deletions faster. The QuikChange Lightning Multi Kit also delivers mutants up to three times faster than our original multi-site mutagenesis kit.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.