XL1-Blue MRF’ and XL1-Blue MR competent cells allow representational cloning of methylated DNA.
Transformation efficiency for XL1-Blue MR and XL-1 Blue MRF’ supercompetent cells of =1 x 10^9 transformants/µg
Transformation efficiency for XL1-Blue MRF´ Kan supercompetent cells of >1 x 10^9 transformants/µg
Transformation efficiency for XL1-Blue MRF’ electroporation competent cells of >1 x 10^10 transformants/µg XL-1
Blue MRF’ and XL-1 Blue MR competent cells allow high efficiency and representational cloning of methylated DNA. All known E. coli K12 restriction systems have been deleted. Use XL1-Blue MRF’ cells when cloning methylated cDNA or genomic DNA, or when cloning methylated PCR products.
When the F’ episome and blue-white screening are unnecessary, use XL1-Blue MR supercompetent cells. When cloning tetracycline-resistant plasmids, use XL1-Blue MRF’ Kan supercompetent cells to select for the F’ episome and provide a more intense blue color for blue-white screening.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.