Competent cells used to prepare plasmid or phagemid cells for more accurate analysis
High quality competent cells for preparing methylation-free DNA.
The SCS110 strain lacks both dam and dcm activity, and DNA propagated in this strain can be digested by methylation-sensitive enzymes such as Xba I, Cla I and EcoR II.;JM110 cells contain the lacIqZ?M15 gene on the F´ episome, allowing blue-white screening for recombinant plasmids.
Most E. coli hosts contain both DNA adenine methylation (dam) and DNA cytosine methylation (dcm) genes. These genes code for proteins that methylate specific sequences when DNA is propagated, making subsequent digestion with methylation-sensitive restriction enzymes impossible. The SCS110 strain, lacks both dam and dcm activity. DNA propagated in this strain can be digested by methylation-sensitive enzymes such as Xba I, Cla I and EcoR II.;The JM110 strain is useful for preparing plasmid or phagemid DNA that is free of Dam or Dcm methylation so that the DNA can be restriction digested by one or more methylation-sensitive restriction enzymes. JM110 is deficient for two methylases (Dam and Dcm) found in most strains of E. coli. Dam methylase recognizes the DNA sequence GATC and methylates the adenine residue at the N-6 position, while Dcm methylase recognizes the DNA sequence CCAGG and CCTGG and methylates the internal cytosine at the C-5 position. JM110 cells contain the lacIqZ?M15 gene on the F´ episome, allowing blue-white screening for recombinant plasmids.
This product is only available to U.S. Domestic Customers.
For research use only, not to be used in diagnostic procedures.