Kit contents:
- Monkeypox specific primer/probe mix
- Monkeypox positive control template
- Internal extraction control primer/probe mix
- Internal extraction control DNA
- Endogenous control primer/probe mix
- RNase/DNase-free water
- Template preparation buffer
The Primerdesign genesig Advanced Kit for Monkeypox is designed for the quantitative and qualitative detection of the monkeypox genome from DNA extracted from human serum, lesion exudate, or scab samples from human/animal patients of all ages. The patient may be exhibiting signs and symptoms of monkeypox virus infection at any point during infection or be being screened after identification as a potential close contact.
The Primerdesign genesig Advanced Kit for Monkeypox is configured for use with any real-time PCR instrument equipped to detect amplification in the FAM and VIC/HEX channels.
The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences of all Monkeypox viral clades available at the time of design (May 2022).
The dynamics of genetic variation mean that new sequence information may become available after the initial design. Primerdesign periodically reviews the detection profiles of our kits and when required releases new versions.