The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. FastStart Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable, recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. An optimized PCR buffer system and a GC-RICH Solution to enable the enzyme to handle a wide range of templates are also supplied.
Features and Benefits
Volume activity: 5 U/μl
Each dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix.
- Higher specificity, sensitivity, and yield:
Hot start PCR makes PCR setup easier - Use robotic setup.
Use this enyzme mix stable for 24 hours at +15 to +25°C - Prevent PCR carryover contamination.
Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes - Cost-effective.
Use the convenient premixed solution of PCR grade nucleotides