Tris-HCl is an excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Grade: Ultra Pure
Purity: ≥99%
Application Areas: Nucleic Acid Gel Electrophoresis & Blotting; Nucleic Acid Electrophoresis; Protein Electrophoresis & Blotting
Product Type: Biochemicals
Biochemical Category: Buffers
Chemical Class: Buffers
Melting Point: 145 - 158°C
UV/Visible Absorbance: OD260 nm (5% aq soln) <0.1 ; OD280 nm (5% aq soln) <0.1
Presentation: White Crystalline Powder
Format: Crystalline Powder
pH: 4.0 - 5.0 (5% aq soln); Useful pH range 7.0 - 9.0(Lit.)
pKa: 8.1 (25°C)(Lit.)
Detection Method: FTIR
Heavy Metals: <5 ppm
Solubility: Solubility (5% aq soln) Clear, Colorless Solution.
Storage & Handling: Store at Room Temperature (15-30°C). Desiccate.