Tris-Acetate/SDS running buffer is used to resolve high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels
Features
- Tris-Acetate/SDS running buffer is compatible with Tris/Acetate gels
- Separate high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels.
- Tris-Acetate system operates at significantly lower operating pH of 8.1 during electrophoresis when compared to traditional Laemmli system thus enabling better band resolution
- Tris-Acetate/SDS running buffer [1X] pH is 8.2
- 1 X concentarion: 50 mM Tricine, 50 mM Tris Base, 0.1% SDS
Application(s:)
- Tris-Acetate/SDS running buffer is used to resolve high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels