HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Synonyms: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
Molecular Formula: C8H18N2O4S
Molecular Weight: 238.3
Linear Structural Formula: C8H18N2O4S
MDL Number: MFCD00006158
Purity: >=99.5% (titration)
Application: HEPES has been used:
•To supplement Dulbecco's modified Eagle's medium to culture and maintain cell lines
•As a component of platelet suspension buffer
•To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
•As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
•For the adjustment and maintenance of pH of biological solutions
•As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
•For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
•As a component of keratinocyte and fibroblast culture medium
Other Notes: Easily compare specifications for HEPES products with the HEPES specification table.
Abs.: A0.1M/280, H2O ≤0.05
Assay: ≥99.5% (titration)
Cation Traces: heavy metals (as Pb): ≤5 ppm
Foreign Activity: DNase, RNase, NICKase, protease, none detected
Grade: Biotechnology Performance Certified
Impurities: endotoxin and total aerobic microbial count, tested
pKa (25°C): 7.5
Quality Level: ELITE
Suitability: cell culture tested
Useful pH Range: 6.8 - 8.2