This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Phosphate EDTA (TPE) consists of 0.9 M Tris-Phosphate, 0.02 M EDTA at a pH of 8.0. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.