MDL No.: MFCD00162417
Storage: -20C
UNSPSC Code: 12352203
General description: IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines,,. The antibody is isolated from goat anti-human IgA antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the alpha chain of human IgA. Goat antihuman IgA is conjugated to peroxidase and purified to remove unconjugated material.Specificity of the peroxidase conjugated anti-human IgA is determined by immunoelectrophoresis (IEP) and cross-reactivity of the conjugate is determined by ELISA. The conjugate is specific for human IgA when tested against human IgA, IgG, IgM, Bence Jones Kappa and Lambda myeloma proteins. The antibody conjugate does not react with mouse or rat IgG.
Application: Peroxidase-conjugated goat anti-human IgA (alpha chain specific) was used in ELISA assays to detect antibodies to G. Lamblia in human saliva. Saliva was diluted in carbonate buffer and anti-human IgA was used at a concentration of 1:1,600. The antibody was detected using o-phenylenediamine dihydrochloride (Sigma) as a substrate.
Application: Anti-human IgA (alpha-chain specific)-peroxidase antibody can be used in dot blot and western blotting.
Application: Anti-human IgA (alpha-chain specific)-peroxidase antibody may also be used for immunohistochemistry using formalin-fixed, paraffin-embedded sections at a dilution of 1:100.
Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Enzyme-linked immunosorbent assay (1 paper)
Immunogen: Purified human IgA.
Physical form: Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal as preservative
Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
RIDADR: NONH for all modes of transport
WGK Germany: 3