Immunogen: Polypeptide sequence of Staphylococcus alphahemolysin targeting the N terminus of the mature toxin.
Description: Mouse monoclonal antibody (mAb) reactive to full length Staphylococcus aureus alpha-hemolysin (alpha-toxin). The antibody detects the antigen produced by S. aureus strains in Western Blotting and ELISA and neutralizes the hemolytic activity of the toxin.
Supplied: 100 µg is supplied in PBS at a concentration of 1.68 mg/mL.
Purification: Antibody is purified using immobilized protein A.
Clonality: Not determined
Relevance: The antibody can be used for detection and neutralization of alpha-toxin produced by virulent strains of S. aureus.
Recommended Dilutions: Optimal use dilution should be determined for each individual application.
Storage: 2-3 weeks +4°C, -20°C long term.
Cross Reactivity: This antibody does not appear to cross react with Staphylococcal enterotoxin B (SEB), rLukS-PV or rLukF-PV based on historical ELISA data. As with most antibodies, this antibody interacts with Protein A in S. aureus culture supernatant via the Fc region.
Toxin neutralization: Using a rabbit RBC lysis assay, EC50 of this antibody for neutralization of 0.3 ug/mL of alpha-toxin was determined to be 0.676 ug/mL.
Intended for research use only, not for human, therapeutic, or diagnostic applications.