This immunoglobulin was prepared from mouse serum using current chromatographic techniques. A quantitative protein analysis as well as immunoelectrophoresis has been applied to determine identity of the product.
Mouse IgG is useful for structural and biological studies, for use as reference standards or as starting material for the preparation of immunogen. Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Mouse IgG is purified from normal mouse serum.
Reconstitution: Reconstitute with deionized or distilled water.
Purity: > 95% by SDS-PAGE
Application Areas: Immunoassays
Product Type: Antibodies
Antibody Type: Secondary Antibodies
Format: It is supplied as purified immunogen in either Tris or phosphate buffered saline.
Formulation: Each vial contains 10 mg of lyophilized protein based on; E1%280 nm = 14. This product was lyophilized from 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3. There is no preservative added.
Specificity: By immunoelectrophoresis: Antibody vs. rabbit anti-mouse serum - shows one band. The product is mouse IgG with no trace of IgA, IgM, or non-immunoglobulin serum proteins detected.
Storage & Handling: Store the lyophilized product at 2 - 8°C. For long-term storage, after reconstitution, aliquot and store frozen at or below -20°C. Avoid repeated freeze/thaw cycles.