Immunogen: The human antibody KZ52 was derived from a human convalescent patient who survived an Ebola virus (EBOV) infection. KZ52 is directed towards the EBOV glycoprotein (GP).
Description: Protein A purified neutralizing human monoclonal antibody reactive to EBOV GP (Parren et al). The antibody detects recombinant EBOV GP without the transmembrane region (EBOV rGPΔTM) expressed in both mammalian and insect cells.
Supplied: 100 µg is supplied in buffer containing Histidine, sodium chloride and sucrose at a concentration of 1.732 mg/mL. No preservative is added.
Purification: Antibody is purified using immobilized protein A.
Clonality: Human variable, human constant of the IgG1 isotype.
Relevance: The antibody can be used for detection of EBOV GP.
Recommended Dilutions:
ELISA: Assay-dependent dilution. Internal QC demonstrates good detection of recombinant EBOV GP coated to a microtiter plate at 1 µg/mL when using KZ52 mAb at a concentration between 10 – 0.001 µg/mL.
Neutralization Assay: KZ52 lot 1605006 showed similar neutralization activity compared to KZ52 reference lot 1603003.
WB: Not suitable for Western Blot analysis.
Storage: 2-3 weeks +4°C, -20°C long term. Avoid repeated freeze/thaws.
Cross Reactivity: No cross-reactivity was observed to Sudan Virus (SUDV) or Marburg Virus (MARV) GP or virus-like particles (VLPs).
Intended for research use only, not for human, therapeutic, or diagnostic applications.