Immunogen: Venezuelan equine encephalitis virus replicons encoding SUDV GP and gamma-radiationinactivated SUDV-Boniface were used to generate the original mouse monoclonal antibody.
Description: Purified mouse monoclonal antibody 16F6 that binds to a conformation epitope on Sudan virus (SUDV) glycoprotein (GP). This antibody demonstrates protection against SUDV both in vitro and in vivo (Dias et al., 2011 Nov 20, Nat Struct Mol Biol. 18 (12): 1424-1427).
Supplied: 100 µg is supplied in buffer containing Histidine, sodium chloride and sucrose at a concentration of 3.764 mg/mL. No preservative is added.
Clonality: Monoclonal of the IgG1 isotype
Relevance: The antibody can be used as a positive control for plaque reduction neutralization test (PRNT) and neutralization assay using SUDV-GP pseudotyped VSV.
Recommended Dilutions:
ELISA: Assay-dependent dilution. Using recombinant GP is not recommended as a coating antigen in ELISA.
WB: Not recommended because 16F6 recognizes conformational epitope.
Storage: 2-3 weeks +4°C, -20°C long term.
Cross Reactivity: In ELISA: No cross-reactivity was observed to Ebola virus (EBOV) or Marburg virus (MARV) virus-like particles (VLP).
Intended for research use only, not for human, therapeutic, or diagnostic applications.