Immunogen: purified recombinant Ebola virus VP40 (EBOV rVP40) produced in E. coli.
Description: Protein G purified mouse monoclonal antibody reactive to EBOV VP40. The antibody detects VP40 in virus-like particles (VLP) and recombinant protein.
Supplied: 100 µg is supplied in buffer containing Histidine, sodium chloride and sucrose at a concentration of 0.718 mg/mL. No preservative is added.
Purification: Antibody is purified using immobilized protein G.
Clonality: Monoclonal of the IgG1 isotype
Relevance: The antibody can be used for detection of EBOV VP40.
Recommended Dilutions:
ELISA: Assay-dependent dilution
Western Blot: Assay-dependent dilution. Internal QC demonstrates good detection when using 3G5 mAb at a use dilution of 10 ng/mL.
Storage: 2-3 weeks +4°C, -20°C long term
Cross Reactivity: Cross reactivity was observed against Sudan Virus (SUDV) VP40 in VLP and SUDV rVP40. No cross reactivity was observed with Marburg Virus (MARV) VP40 in VLP or MARV rVP40.
Intended for research use only, not for human, therapeutic, or diagnostic applications.