Rabbit IgG polyclonal antibody for IL10 detection. Tested with WB, Direct ELISA in Human;Mouse;Rat.
Background:
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Attributes:
sku: A00021-2
name: Anti-IL10 Picoband™ Antibody
gene_name: Il10
clonality: Polyclonal
concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
size: 100μg/vial
uniprot_id: P22301
host: Rabbit
immunogen: E. coli-derived human IL10 recombinant protein (Position: S19-N178).
form: Lyophilized
storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
cross_reactivity: No cross reactivity with other proteins.
reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
application_details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human
applications: ELISA, WB
reactivity: Human, Mouse, Rat
research_category: Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
synonyms: Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10
gene_full_name: interleukin 10
protein_function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
subcellular_localization: Secreted.
tissue_specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
recommended_detection_systems: Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.