Polyclonal antibody for APP detection. Host: Rabbit.Size: 100μL. Tested applications: IF. Reactive species: Human. APP information: Molecular Weight: 86943 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit. Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. Gamma-CTF(59) peptide is located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with APBB1 (Fe65). Beta-APP42 associates with FRPL1 at the cell surface and the complex is then rapidly internalized. APP sorts to the basolateral surface in epithelial cells. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body. Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment. Associates with GPC1 in perinuclear compartments. Colocalizes with SORL1 in a vesicular pattern in cytoplasm and perinuclear regions; Tissue Specificity: Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T- lymphocytes. Appican is expressed in astrocytes.
Attributes:
sku: A00081-2
name: Anti-Beta Amyloid Antibody
gene_name: APP
clonality: Polyclonal
concentration: 1.0mg/mL by UV absorbance at 280 nm
contents: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
size: 100μL
uniprot_id: P05067
host: Rabbit
immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the amino terminus (aa 1-14) of human beta amyloid conjugated to KLH using maleimide.
form: Liquid
storage: Store at -20°C for one year, at 4°C for one month. We suggest the antibody be aliquotted into small vials and stored frozen at -20°C upon receiving. Avoid repeated freezing and thawing.
application_details: Western Blot, 1:1, 000-1:5000
Immunohistochemistry: 1:50-1:200
IF Microscopy: 1:50-1:200
ELISA : 1:10,000 - 1:30,000
applications: ELISA, IF, IHC, WB
reactivity: Human, Mouse
research_category: Adapters, Apoptosis, Associated Proteins, Cell Biology, Cytoplasmic, Developmental Biology, Intracellular, Nervous System Development, Neural Signal Transduction, Neurogenesis, Neurology Process, Neuroscience, Organelle Proteins, Organogenesis, Protein Trafficking, Signal Transduction
synonyms: Amyloid beta A4 protein;ABPP;APPI;APP;Alzheimer disease amyloid protein;Cerebral vascular amyloid peptide;CVAP;PreA4;Protease nexin-II;PN-II;N-APP;Soluble APP-alpha;S-APP-alpha;Soluble APP-beta;S-APP-beta;C99;Beta-amyloid protein 42;Beta-APP42;Beta-amyloid protein 40;Beta-APP40;C83;P3(42);P3(40);C80;Gamma-secretase C-terminal fragment 59;Amyloid intracellular domain 59;AICD-59;AID(59);Gamma-CTF(59);Gamma-secretase C-terminal fragment 57;Amyloid intracellular domain 57;AICD-57;AID(57);Gamma-CTF(57);Gamma-secretase C-terminal fragment 50;Amyloid intracellular domain 50;AICD-50;AID(50);Gamma-CTF(50);C31;APP;A4, AD1;
gene_full_name: Amyloid beta A4 protein
molecular_weight: 86943 MW
protein_function: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER- dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. .
subcellular_localization: Membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit. Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. Gamma-CTF(59) peptide is located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with APBB1 (Fe65). Beta-APP42 associates with FRPL1 at the cell surface and the complex is then rapidly internalized. APP sorts to the basolateral surface in epithelial cells. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body. Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment. Associates with GPC1 in perinuclear compartments. Colocalizes with SORL1 in a vesicular pattern in cytoplasm and perinuclear regions.
tissue_specificity: Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T- lymphocytes. Appican is expressed in astrocytes. .