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Jackson Immunoresearch

Alexa Fluor® 488 ChromPure Mouse IgG, F(ab')2 fragment

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ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals.

Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.

Host: Mouse

Product: IgG, F(ab')2 fragment

Conjugate: Alexa Fluor® 488

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateOne year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityBased on immunoelectrophoresis at an antigen concentration of 20 mg/ml, the pattern of precipitation against goat anti-mouse whole serum is the same as that against goat anti-mouse IgG, F(ab')2 fragment specific. No precipitin line was detected against goat anti-mouse IgG, Fc fragment specific. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative0.05% Sodium Azide 

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Product Detail
Thomas No.
C840F62
Mfr. No.
015-540-006
Description
Alexa Fluor® 488 ChromPure Mouse IgG, F(ab')2 fragment, 1 mg
list price/quantitytotal
$0.00
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Product C840F62 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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