Immunogen: Staphylococcal enterotoxin B (SEB)
Description: Mouse monoclonal antibody reactive to Staphylococcus aureus enterotoxin B.
Supplied: 100 µg is supplied in PBS at a concentration of 0.396 mg/mL.
Purification: Antibody is purified using immobilized protein G.
Clonality: Monoclonal of the IgG1 subtype
Relevance: The antibody can be used for capture of SEB in an ELISA sandwich assay and Western blot detection. It is not recommended for direct detection in an ELISA.
Recommended Dilutions:
?ELISA: Assay-dependent dilution. Internal QC demonstrates good capture of purified SEB using this monoclonal antibody coated to a plate at 1.0 µg/mL.
WB: Antibody can be utilized for Western blot detection of SEB at a use dilution of 1 µg/mL.
Neutralization: 10 nM of 3D10 completely inhibits superantigenic activity of 0.1 ng/ml SEB.
Storage: 2-3 weeks +4°C, -20°C long term.
Cross Reactivity: This antibody does not appear to cross react with Staphylococcal enterotoxin C (SEC1).
Intended for research use only, not for human, therapeutic, or diagnostic applications.