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Jackson Immunoresearch

4 nm Colloidal Gold AffiniPure Goat Anti-Human IgG, Fc? Fragment Specific (LM Grade)

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4 nm Colloidal Gold AffiniPure Goat Anti-Human IgG, Fc? Fragment Specific (LM Grade)

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

Target: Human

Host: Goat

Antibody Format: Whole IgG

Specificity: IgG, Fc? Fragment Specific

Minimal Cross Reactivity: Bovine, Horse, Mouse Serum Proteins

Conjugate: 4 nm Colloidal Gold

Clonality: Polyclonal

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. After rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Use an aliquot within one day of thawing. 
Expiration dateone year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. 
Buffer0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5 
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative0.05% Sodium Azide 
Suggested Working Concentration or Dilution Range
Histo-/Cyto-chemistry- 1:20-1:200
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C840L23
Mfr. No.
109-185-098
Description
4 nm Colloidal Gold AffiniPure Goat Anti-Human IgG, Fc? Fragment Specific (LM Grade), 1.0 ml
list price/quantitytotal
$0.00
$0.00 (0 Items)
Product C840L23 is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account - Click here to login or create your web profile. If you do not have a Thomas Business Account, please Contact Us and someone will respond with details on how to apply for a business account with us.

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