Immunogen: Staphylococcus epidermidis, Hay strain (ATCC 55133).
Description: Mouse monoclonal antibody (mAb) reactive to Staphylococcus lipoteichoic acid.
Supplied: 200 µg is supplied in PBS at a concentration of 0.92 mg/mL.
Purification: Antibody is purified using immobilized protein A.
Clonality: Monoclonal of the IgG1 subtype
Relevance: Lipoteichoic acid (LTA) is the major proinflammatory structure present within the cell wall layer of most gram-positive bacteria. It plays an important role in the initiation and progression of bacterial infection, inflammation, and septic shock. The antibody can be used for detection of LTA produced from Staphylococcus cells.
Recommended Dilutions:
Optimal use dilution should be determined for each individual application.
ELISA: 0.1 – 1.0 ug/mL
Opsonization assay: use at 80-160 µg/ml (optimized for Staph. epidermidis, Hay strain).
Storage: 2-3 weeks +4°C, -20°C long term.
Cross Reactivity: Based on ELISA, this mAb reacts with LTA from S. epidermidis, Hay strain (ATCC 55133) as well as clinical strains of S. epidermidis types I, II and III. The antibody also reacts with LTA from S. aureus types 5 and 8, Streptococcus pyogenes, Streptococcus fecaelis and Streptococcus mutans. This mAb does not react with peptidoglycan from S. aureus, peptidoglycan rhamnose or pneumococcal polysaccharides. It also does not react with live E. coli or H. influenzae type B.
Intended for research use only, not for human, therapeutic, or diagnostic applications.