Anti-5mC antibody for immunoprecipitation of methylated DNA; ELISA; immunoblotting; immunofluorescence
- Specifically binds to 5-methylcytosine in ssDNA context.
- No detectable cross reactivity with non-methylated cytosine.
The mouse Anti-5-Methylcytosine Monoclonal Antibody specifically recognizes methylated cytosines. The antibody binds to 5-mC in single-stranded DNA, with no detectable cross reactivity to non-methylated or hydoxymethylated cytosines. This product is ideal for immuno-based assays such as methylated DNA Immunoprecipitation (MeDIP), ELISA and dot blot.
Protein Concentration: 0.5 µg/µl
Source: Mouse
Specificity: 5-Methylcytosine in single stranded DNA from any source including human, rat, mouse, plants, etc.
Buffer Solution: Antibody is provided in PBS (Phosphate Buffered Saline, pH 7.4) with 0.05% Sodium azide as a preservative.
Isotype: IgG1 (κ light chain)
Clone: 7D21
Storage: Store at -20 C with prolonged storage at -80° C. Avoid repeated freeze/thawing