L-histidine is an essential amino acid with a basic side chain, and is hydrophilic in character. It contains an imidazole group in the side chain. Histidine is biosynthesized from ATP, 5-phosphoribosyl-1-pyrophosphate (PRPP), and glutamine. In turn, histidine is degraded to glutamate by histidase, urocanase, and imidazolonepropionase via the formation of urocanate, 4-imidazolone 5-propionate, and N-formiminoglutamate.
Precursor of histamine by action of histidine decarboxylase.
L-Histidine has been used to study cultures of the human T-lymphoblastic leukemia cell line MOLT-4 to study modulation of apoptosis. Exogenous histidine has been shown to enhance the biosynthesis of lovastatin by cultured Aspergillus terreus. Histidine has been utilized as a single nitrogen source to probe swarming in Pseudomonas aeruginosa on agar. It is generally used in media preparation.
Purity: ≥99%
Key Applications: Cell culture component
Product Type: Biochemicals
Biochemical Category: Amino Acids & Peptides
Chemical Class: Biochemicals
Melting Point: 282°C (Lit.)
Optical Rotation: +11.0° to +13.5° (c=10, 4N HCl)
Presentation: Crystalline, Free Base
pH: 7.0 - 8.5 (1% aq soln)
Loss on Drying: ≤0.5%
Solubility: Soluble 50 mg/mL in 0.5 M HCl.
Storage & Handling: Room Temperature