The venous blood from which this product is manufactured has been tested for the presence of Hepatitis B Surface Antigen (HBsAg) and found to be non-reactive by a currently approved FDA test. The blood has also been tested for the presence of Anti-HCV, Anti-HIV-1 and Anti-HIV-2, antibodies and found to be negative. The source donors have been tested in accordance with 21 CFR 640. This blood is non-reactive for syphilis.
This protein is prepared by a modification of the Chen low pH charcoal method. Contains approx. 0.2 mg/g fatty acids.
Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Due to their free hydrophobic region fatty acid free albumins are used to solubilize lipids in tissue culture, and are also used as blocking agents in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis).
Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.
Purity: 100% By cellulose acetate electrophoresis
Concentration: 98.0% By nitrogen analyzer, dry weight basis
Key Applications: Immunoassay, Mammalian Cell Culture
Application Areas: Immunoassays; Mammalian Cell Culture
Product Type: Proteins, Enzymes & Peptides
Protein or Enzyme Type: Albumins
Isoelectric point (pI): (G/2 = 0.15) 4.7
pH: 7.2 (7% solution)
Moisture content: <5% By Karl Fischer
NOTES: Total Lipids: 0.2 mg/g By supercritical fluid extraction Free Fatty Acids: <0.1 mg/g By chromatography Esterified Fatty Acids: <0.1 mg/g By chromatography Sodium: 5.0 mg/g By flame photometer Chloride: 11.5 mg/g By coulometric titrator
Solubility: Soluble in water at 50 mg/mL.
Storage & Handling: Store at 2-8°C.
Certifications: Fatty Acid Free