Agarose is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. Agarose is a purified linear galactan hydrocolloid isolated from agar or agar-bearing marinealgae.
As a gelling agent, agarose is used: 1.) to separate nucleic acids electrophoretically because its gels have larger pore sizes than polyacrylamide gels at low concentrations. Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic); 2.) To demonstrate cross reaction in IEP (Immuno electrophoresis) and Ouchterlony (double diffusion) plates in which antibody-antigen precipitin lines are studied; 3.) to make gel plates or overlays for cells in tissue culture; 4.) To form a gel matrix (either beaded and/or crosslinked) which can be used in chromatographic separations.
Grade: Molecular Biology reagent
Suitability: Ideal for nucleic acid electrophoresis, analysis and purification.
Application Areas: Nucleic Acid Electrophoresis; Nucleic Acid Gel Electrophoresis & Blotting
Product Type: Biochemicals
Biochemical Category: Agaroses & Acrylamides
Chemical Class: Agaroses & Acrylamides
Melting Point: 87.3 - 89.5°C
Presentation: White Powder
Format: Powder
pH: 6 - 7.5 (1% aq soln)
Electroendosmosis (-MR): 0.09 - 0.13
Gel Strength: ≥600 g/cm2 (1.5% gel)
Gel Temperature: 35 - 37°C
Foreign activity: DNase, Rnase and Protease free
Moisture content: ≤10%
NOTES: Sulfur ≤0.15%
Solubility: Soluble in 1.5 g plus 100 mL H2O.
Storage & Handling: Store at Room temperature (15-30°C)