Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility. Suitable for nucleic acid analytical and preparative electrophoresis; blotting; and protein electrophoresis (i.e. Radial Immunodiffusion).
Specifications
- Moisture: ≤ 7%
- Ash: 4%
- EEO: 0.05-0.13
- Sulfate: ≤ 0.1%
- Clarity 1.5% (NTU): ≤ 3
- Gel Strength 1% (g/cm2): ≥ 1200
- Gel Strength 1.5% (g/cm2): ≥ 2500
- Gelling Temperature 1.5% (ºC): 36 ± 1.5
- Melting Temperature 1.5% (ºC): 88 ± 1.5
- DNase/ RNase Activity: None Detected
- DNA Resolution ≥1000bp: Finely resolved
- Gel background: Very low
Appropriate Agarose Concentrations
| GEL CONCENTRATION (%) | 1X TAE BUFFER | 1X TBE BUFFER |
| 0.6 | 1,000-20,000bp | 1,000-15,000bp |
| 0.8 | 500-12,000bp | 500-10,000bp |
| 1.0 | 300-8,000bp | 250-7,000bp |
| 1.2 | 200-6,000bp | 200-5,000bp |
| 1.5 | 100-3,500bp | 100-3,000bp |
| 2.0 | 50-2,000bp | 50-2,000bp |
Features
- Extraordinary mechanical resistance for more reliable and easier handling
- Greater thermal stability due to high hysteresis (difference between gelling and melting temp)
- High transparency and visibility
- Easy preparation
Applications
- Analytical nucleic acid electrophoresis
- Preparative nucleic acid electrophoresis
- Blotting
- Protein electrophoresis such as radial immunodiffusion