Description: Highly Purified lipoteichoic acid (LTA) from Staphylococcus aureus
Size: 5.0 mg supplied as a lyophilized powder.
Relevance: LTA can be found at the cell surface of most gram-positive bacteria. Few pathogen recognition receptors have been reported for LTA and a more precise biological role of LTA is still under investigation. When expressed on the cell exterior, LTA is thought to act as an adhesion as well as mediating inflammatory responses in the host.
ELISA Testing: Highly purified LTA was coated onto microtiter plates at either 1µg/well or 0.2µg/well. Positive control mouse sera or negative control mouse sera were serially diluted 2-fold starting at a 1:200 dilution and incubated on the coated plates. Washed plates were detected with anti-mouse HRP conjugate and TMB substrate. After stopping the reaction with 0.5M HCL, OD450 is reported.
Storage: -4°C
Intended for research use only, not for human, therapeutic, or diagnostic applications.