Description: EcoCRM™ is CRM197 produced as a soluble intracellular protein in E. coli. CRM197 is genetically detoxified diphtheria toxin. Specifically, it has a single amino acid substitution (glycine52 to glutamic acid), which causes the ADPribosyltransferase activity to be lost. CRM197 is widely used as the carrier protein for conjugate vaccines. EcoCRM™ is expressed as a recombinant protein in Escherichia coli.
Supplied by: Fina Biosolutions LLC
Size: 1 mg of protein is supplied at a concentration of 1.0 mg/mL (by absorbance at 280 nm) in 1X PBS, pH 7.6, 10% glycerol. The theoretical molecular weight of the protein is 58.4 kDa.
Purity: >95% by HPLC
OD260/OD280 = 0.57 (< 1.0, the protein is free from nucleic acid contaminant)
Endotoxin: 0.16 EU/µg
Relevance: CRM197 is widely used as the carrier protein for conjugate vaccines. Vaccines for Haemophilus influenzae b, meningococcal disease and Streptococcus pneumoniae use CRM197. Unlike toxoids, CRM197 has its full complement of lysines available for conjugation. It is also thought to cause less interference in multivalent vaccines. EcoCRM™ is stable in PBS.
Storage: -80°C, avoid repeated freeze/thaws
Intended for research use only, not for human, therapeutic, or diagnostic applications.