The bio-World Abundant Protein Removal a-1-antitrypsin kit removes the high-abundant protein a-1-antitrypsin from human serum and plasma. The high affinity-based column contains Separopore® matrix coupled with antibodies against a-1-antitrypsin.
The presence of abundant proteins in human serum and plasma often masks the detection of low-abundant proteins of interest that serve as biomarkers for disease when being studied by 2D gel electrophoresis or LC/MS. After depletion of these high-abundant proteins, serum samples consist of an enriched pool of low-abundant proteins that can be further studied without significant interferences with abundant proteins. The remarkable selectivity provided by the resins and the optimized design of the columns results in background binding of less than 10% to other serum proteins. Sample volumes from 20-60µl can be processed without any loss of selectivity.
Kit Components:
a-1-antitrypsin Ab coupled to Separopore®, 1ml column
Binding/Wash buffer, 25 ml