Protein quantification is often required before precessing protein samples for analysis.The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+to Cu1+by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. It is water-soluble and strongly absorbs light at 562nm in a linear fashion with increasing protein concentration.
BCA Protein Assay
A simple and sensitive colorimetric assay for the determination of protein concentration. The concentration dependent reaction reduces Cu2+ to Cu1+ producing a blue color that is read at 562nm.
Kit Contents
BCA Reagent A : 2x500mL
BCA Reagent B : 25mL
BSA Protein Standard (2mg/ml) :10x1ml
Storage: Should be stored at RT, except for BSA standard at -20°C