Peroxidase-Conjugated Goat IgG Fraction To Human Complement C3 is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human complement C3 and buffer salts.
Background Information
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacteria infection.
Applications
Peroxidase-Conjugated Goat IgG Fraction To Human Complement C3 has been used in ELISA, Immunohistochemistry, Flow Cytometry, Immunoprecipitation and Western Blotting. It is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Typical Working Concentration
Dilution Recommended: 1:500 - 1:4,000 for Tissue Staining; 1:1,000 - 1:8,000 for Immuno Blotting; 1:2,000 - 1:16,000 for ELISA/FIA
Preparation Method
Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Analysis Note
The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with human complement C3. The product is tested for purity and specificity at final concentration by immunoelectrophoresis.