Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to human albumin and buffer salts.
Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining.
Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with human albumin. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected. It shows reactivity to human albumin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
DILUTION RECOMMENDATIONS:
- 1:500 - 1:4,000 for Tissue Staining
- 1:1,000 - 1:8,000 for Immuno Blotting
- 1:2,000 - 1:16,000 for ELISA/FIA
Reconstitute product with 2.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.
Store lyophilized product at 2-8°C. For long-term storage, aliquot and freeze reconstituted product at -20°C or lower.