Peanut agglutinin (PNA, Peanut Agglutinin) is a 110,000 molecular weight lectin composed of four identical subunits of approximately 27,000 daltons each. PNA binds preferentially to a commonly occurring structure, galactosyl (b-1,3) N-acetylgalactosamine. This carbohydrate sequence (called the “T-antigen”) is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids.
PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding has been employed as a measure of cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte human subpopulations and animals, and to measure the levels of lymphoid cell populations in many diseases. Presence of Ca++ can enhance PNA binding.
Specifications:
- Form: Supplied in 1% NaCl containing 0.04% sodium azide as preservative.
- Matrix: 4% cross-linked agarose beads.
- Ligand source: Arachis hypogaea (peanut lectin).
- Ligand coupling method: CNBr activation.
- Ligand Concentration: 5 mg Peanut lectin/ml drained gel.
- Binding Capacity: 0.5mg/ml.
- Inhibiting/Eluting Sugar: 200 mM galactose.
- Carbohydrate for Elution: 0.1M lactose or 0.2M galactose in buffer. 10-50mM N-acetylgalactosamine may also be used.
Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.