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Protein G-Separopore® (Agarose) 4B-CL
- Protein G binds specifically to Fc regions of many mammalian immunoglobulins
- Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids
- These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments
- Affinity matrix with immobilized protein G (from Streptococcus sp.) by CNBr activation coupling method
- Protein G-Separopore® 4B-CL is used for purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG
Ligand: | Native Protein-G from Streptococcus sp. |
Matrix: | Separopore® 4B-CL (crosslinked agarose beads, 4%) |
Matrix activation: | Cyanogen bromide |
Particle size range: | 52 – 165 µm |
Exclusion limit range: | 6 x 104 - 2 x 107 |
Ligand density: | ~ 2 mg Protein-G / ml drained medium |
Binding capacity: | ~ 20 mg human IgG / ml drained gel |
pH stability: | 3 – 9 (for use), 2 – 10 (for cleaning) |
Storage: | 2 – 8°C |
Supplied as suspension in 0.5M NaCl |
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Product Detail
- Description
- Protein G Separopore® 4B-CL, 1 mL
- Packaging Size
- 1 mL
- UN Number
- 41115700
list price/quantity | total | | $0.00 |
- Description
- Protein G Separopore® 4B-CL, 5 mL
- Packaging Size
- 5 mL
- UN Number
- 41115700
list price/quantity | total | | $0.00 |
- Description
- Protein G Separopore® 4B-CL, 10 mL
- Packaging Size
- 10 mL
- UN Number
- 41115700
list price/quantity | total | | $0.00 |
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account -
Click here to login or
create your web profile. If you do not have a Thomas Business Account, please
Contact Us and someone will respond with details on how to apply for a business account with us.