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Epoxy-activated Separopore® (Agarose) 4B-CL
- Used in coupling of hydroxyl, amino or thiol groups to Separopore® 4B via a 12-carbon hydrophilic spacer arm
- Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group
- The matrix provides a 10-atom spacer arm convenient for coupling of small ligands
- Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates
- Epoxy-activated Separopore® 4B is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group
- Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules
| Matrix active group: | Epoxy |
| Matrix: | Separopore® 4B-CL (crosslinked agarose beads, 4%) |
| Particle size range: | 52 – 165 µm |
| Group to be coupled: | -NH2, -OH, or -SH |
| Molecular weight range: | 6 x 104 – 2 x 107 |
| Chemical linkage of ligand: | Ether |
| Matrix spacer: | 12 atoms (when ligands are coupled to free epoxy groups. Linkage is stable, uncharged ether) |
| Coupling conditions: | pH 9 – 10 (amino ligands), pH 11 – 12 (hydroxyl ligands), pH 7.5 - 8.5 (thiol ligands); Temp: 4 – 37 °C; Time: 16 h to several days; organic solvents possible |
| pH stability: | 2 – 11 (ligand dependent) |
| Storage: | 2 – 8 °C |
| Supplied as lyophilized powder stabilized with lactose and dextran |
| 1g swells to 3 – 4 ml |
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Product Detail
- Description
- Epoxy-Activated Separopore® 4B-CL, 1 g
- Packaging Size
- 1 g
- UN Number
- 41115700
| list price/quantity | total | | $0.00 |
- Description
- Epoxy-Activated Separopore® 4B-CL, 5 g
- Packaging Size
- 5 g
- UN Number
- 41115700
| list price/quantity | total | | $0.00 |
- Description
- Epoxy-Activated Separopore® 4B-CL, 10 g
- Packaging Size
- 10 g
- UN Number
- 41115700
| list price/quantity | total | | $0.00 |
- Description
- Epoxy-Activated Separopore® 4B-CL, 25 g
- Packaging Size
- 25 g
- UN Number
- 41115700
| list price/quantity | total | | $0.00 |
Product is restricted and can only be purchased by customers with a web profile linked to a Thomas Business Account -
Click here to login or
create your web profile. If you do not have a Thomas Business Account, please
Contact Us and someone will respond with details on how to apply for a business account with us.