Cyanogen bromide-activated Separopore® 4B matrix is used for preparation of resins for affinity chromatography. Pre-activated medium is suitable for immobilization of ligands containing primary amines. Cyanogen bromide (CNBr) in base reacts with hydroxyl groups on Separopore® 4B to form cyanate esters or imidocarbonates. These groups react readily with primary amines under very mild conditions; the net result is a covalent coupling of a ligand to the Separopore® matrix.
- Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines
- Multi-point attachment of many protein ligands, resulting in a chemically stable product
- Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies