Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localized proteins have been shown to be involved in intracellular membrane trafficking and signaling.FOCUS™ Signal Proteins kit fractionates lipid raft localized proteins from other cellular proteins by employing non ionic detergents. Signal Protein Extraction Buffer is a proprietary formulation of non-ionic detergents designed to efficiently extract and remove soluble proteins, leaving lipid rafts containing signal proteins as a detergent insoluble fraction. The resulting rafts are then solubilized in FOCUS™ Protein Solubilization Buffer, a supplied 2D electrophoresis compatible buffer or a different buffer of choice. FOCUS™ Signal Proteins kit is designed for >50 preps, where one prep is:
- 100mg mammalian tissue
- 50µl wet animal cell pellet
- 50µl wet yeast pellet
- 50µl wet bacteria pellet
- 250mg plant tissue
Features
- Fractionate lipid raft localized proteins.
- A 2D electrophoresis compatible buffer is provided.
Applications
- Isolate signal proteins that are localized to lipid rafts.
- Study movement of activated proteins to and from lipid rafts.
- Suitable for 2D gel electrophoresis, proteomics and cell signaling studies.