FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localized proteins have been shown to be involved in intracellular membrane trafficking and signaling.
FOCUS™ Global Fractionation is designed for >50 preps, where one prep is:
- 100mg mammalian tissue
- 50µl wet animal cell pellet
- 50µl wet yeast pellet
- 50µl wet bacteria pellet
- 250mg plant tissue
Features
- Fractionate integral, peripheral and lipid raft associated proteins.
- Fractionation of complex proteomes into multiple, specific fractions.
Applications
- Selective fractionation of membrane proteins from tissues, cells, plants, yeast, bacteria, insects, and other sources.
- Fractionation of membrane proteins from lipid rafts.
- Suitable for membrane protein preparation for 2D gel electrophoresis and other applications.
- Suitable for proteomics and cell signaling studies.