HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from bacterial cultures.The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity of the protein. The GST tagged protein is purified by affinity chromatography by adding 0.5ml glutathione resin to the clarified lysate.The resin is transferred to a convenient spin column, where it is rapidly washed and the GST protein is eluted.
Bacterial- PELB™ kit has been developed for the extraction of soluble proteins from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The Bacterial-PE LB™ lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal force.
HOOK™ GST Protein Spin Purification kit is optimized to yield ~1mg/50ml culture of soluble GST tagged protein, with a purity of 80-90%, dependent on expression levels, resin type, conformation and solubility characteristics of the protein.
For larger scale GST protein isolation from bacteria we recommend our gravity flow kits for >10mg protein from ~250ml bacteria.
Features
- Isolate >1mg GST tagged proteins from ~50ml bacterial culture
- No sonication required, supplied with lysis buffer and enzyme
- Spin column format
Applications
- Spin column format for the isolation of GST-tagged recombinant proteins from bacteria