The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5µl to 4ml sample volumes. Also available in 96-well spin plate formats for processing up to 96 samples. The SpinOUT™ columns are available in three MWCO sizes for >700, >6,000 or >30,000 Dalton peptides or proteins and are suitable for samples containing as little as 20µg protein/ml. The SpinOUT™ columns are easy to use; simply apply the protein sample and centrifuge to recover proteins and nucleic acids with the column retaining more than 95% of the salts and small molecules (<100Da for SpinOUT™ GT-100, <1,000Da forSpinOUT™GT-600 and <1,500Da forSpinOUT™GT-1200). Spin-OUT™ GT-100 is for the purification of peptides and proteins >700Da. Spin-OUT™ GT-600 is for the purification of proteins >6kDa and nucleic acids larger than 10bp. Spin-OUT™ GT-1200 is for the purification of proteins >30kDa and removal of molecules >1,500Da. The columns are ideal for separating proteins from peptides.
Five sizes are available for each MWCO and are highlighted below:
| Cat. # | Description | # Supplied | Resin Bed Volume (ml) | Sample Load Volume (ml) |
| 786-865 | SpinOUT™ GT-100, 0.1ml | 25 | 0.1 | 0.005-0.02 |
| 786-866 | SpinOUT™GT-100, 1ml | 10 | 1 | 0.05-0.1 |
| 786-867 | SpinOUT™GT-100, 3ml | 10 | 3 | 0.1-0.5 |
| 786-868 | SpinOUT™GT-100, 5ml | 5 | 5 | 0.5-2 |
| 786-869 | SpinOUT™GT-100, 10ml | 5 | 10 | 0.5-4 |
| 786-703 | SpinOUT™ GT-600, 0.1ml | 25 | 0.1 | 0.005-0.02 |
| 786-170 | SpinOUT™GT-600, 1ml | 10 | 1 | 0.05-0.1 |
| 786-171 | SpinOUT™GT-600, 3ml | 10 | 3 | 0.1-0.5 |
| 786-704 | SpinOUT™GT-600, 5ml | 5 | 5 | 0.5-2 |
| 786-705 | SpinOUT™GT-600, 10ml | 5 | 10 | 0.5-4 |
| 786-706 | SpinOUT™GT-1200, 0.1ml | 25 | 0.1 | 0.005-0.02 |
| 786-172 | SpinOUT™GT-1200, 1ml | 10 | 1 | 0.05-0.1 |
| 786-173 | SpinOUT™GT-1200, 3ml | 10 | 3 | 0.1-0.5 |
| 786-707 | SpinOUT™GT-1200, 5ml | 5 | 5 | 0.5-2 |
| 786-708 | SpinOUT™GT-1200, 10ml | 5 | 10 | 0.5-4 |
Also available in 96-well spin plate formats for processing up to 96 samples.
For the desalting and clean up of PCR reactions, click here .
Features
- Offered in 3 MWCO sizes: >700Da,>6kDa and >30kDa proteins
- Available in 5 sizes for sample volumes of 5µl to 4ml
- 96-well plate formats available
- Easy-to-use, spin-format columns for rapid purification
Applications
- Protein purification and contamination removal from samples
- For the desalting and buffer exchange of protein and nucleic acid solutions
- Separate proteins from peptides
- Desalt peptides