G-Trap™ Ion Exchange Selection Kit contains 4 different types of 1 ml G-Trap™ Ion Exchange Agarose Fast Flow Columns which are used for initial screening of the suitable ion exchange resin and /or pH or ionic strength optimization of start and elution buffer to separate the target protein or substance via ion exchange chromatography.
Biomolecules or proteins that carry net charge opposite to that of ion-exchanger bind to that ion exchanger and are thus separated from bulk molecules in the sample.
G-Trap™ Ion Exchange Selection Kit contains one of each, G-Trap™ CM Agarose Fast Flow , G-Trap™ SP Agarose Fast Flow , G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow column .
Once initial screening for suitable ion exchange resin is done byG-Trap™ Ion Exchange Selection Kit, indiviaulG-Trap™ Ion Exchange Agarose Fast Flow Column can be ordered in bulkas per requirement.
G-Trap™ Ion Exchange Agarose Fast Flow Columns are available in 1 ml and 5 ml Column Volume namely G-Trap™ CM Agarose Fast Flow , G-Trap™ SP Agarose Fast Flow , G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose Fast Flow column ..
G-Trap™ Ion Exchange Agarose Fast Flow Columns are ready to use prepacked columns for separation of biomolecules on the basis of charge. G-Trap™ Ion Exchange Agarose Fast Flow Columns are used for method scouting, separation or purification of biomolecules, sample concentration and sample cleanup for charged molecules.
Theproperties of the G-Trap™ Ion Exchange Selection Kit resin and the column are listed below
G-Trap™ Ion Exchange Selection Kit resin
G-Trap™ CM Agarose Fast Flow resin
Ion Exchanger: Weak cation exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand : Carboxymethyl
Ionic Capacity: 0.09-0.13 mmol (H+)/ml
Binding Capacity: 50 mg lysozyme/ ml medium
pH stability Working Range: 4-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ SP Agarose Fast Flow resin
Ion Exchanger: Strong cation exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand : Sulphopropyl
Ionic Capacity: 0.18-0.25 mmol (Na+)/ml
Binding Capacity: 70 mg lysozyme /ml medium
pH stability Working Range: 2-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol containing 0.2M sodium acetate
G-Trap™ DEAE Agarose Fast Flow resin
Ion Exchanger: Weak anionexchanger
Matrix:Cross-linked agarose beads, 6%
Bead size:45-165 µm
Ligand :Diethylaminoethyl
Ionic Capacity: 0.11-0.16mmol (Cl-)/ml
Binding Capacity: 90mg HSA/ml medium
pH stability Working Range: 2-9
pH stability Cleaning-in-Place (CIP):2-14
Maximum Flow Velocity:450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure:0.3MPa
Exclusion limit(globular proteins):4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions:4 to 30°C, 20% Ethanol
G-Trap™ Q Agarose Fast Flow resin
Ion Exchanger:Strong anion exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand :Quaternary ammonium
Ionic Capacity: 0.18-0.25mmol (Cl-)/ml
Binding Capacity: 120 mg HSA /ml medium
pH stability Working Range: 2-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol
G-Trap™ Ion Exchange Selection Kit, 1 ml columns
Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)
Application(s):
- G-Trap™ Ion Exchange Selection Kit contains 4 different types of 1 ml G-Trap™ Ion Exchange Agarose Fast Flow Columns which are used for initial screening of the suitable ion exchange resin and /or pH or ionic strength optimization of start and elution buffer to separate the target protein or substance via ion exchange chromatography. Biomolecules or proteins that carry net charge opposite to that of ion-exchanger bind to that ion exchanger and are thus separated from bulk molecules in the sample.