The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat brains.
The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.
Features
Blot lane order:
- Protein marker
- Rat Frontal Cortex Lysate
- Rat Posterior Cortex Lysate
- Rat Cerebellum Lysate
- Rat Hippocampus Lysate
- Rat Olfactory Bulb Lysate
- Rat Striatum Lysate
- Rat Thalamus Lysate
- Rat Midbrain Lysate
- Rat Entorhinal Cortex Lysate
- Rat Pons Lysate
- Rat Medulla Lysate
- Rat Spinal Cord
- Rat Whole Brain Lysate