The blot is prepared using total protein extracted from a variety of 5-10 day old, whole plant tissues.
The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer .
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.
Features
Blot lane order:
- Protein marker
- Green Algae Lysate
- Yeast Lysate
- Barley Lysate
- Sweet Corn Lysate
- Rye Grass Lysate
- Oat Lysate
- Wheat Lysate
- Soy Lysate
- Mung Lysate
- Sunflower Lysate
- Cotton Lysate
- Beet Lysate
- Tomato Lysate
- Spinach Lysate