- For use following cell lysis measures including lysis reagents, grinding via micropestle, and shearing via passing the lysate through a 20-G needle
- Designed to filter out the excess tissue materials and contaminants prior to the DNA binding step
IBI Replacement MINI Genomic DNA Filter Columns and Collection Tubes have been designed specifically for purifying Genomic DNA from plant samples. These columns can be used to utilize excess reagents from other DNA purification products that also use a lysis method. Protocols from the original, complete kit (regardless of brand) may be used with these columns as well. (Note: These are NOT DNA-binding columns.)
IBI Genomic DNA Filter Columns and Collection Tubes are direct replacements for the filter columns in the following IBI genomic DNA extraction kit: MINI Genomic DNA Kit for Plant (IB47240, IB47241).
The quality of the IBI MINI Genomic DNA Filter Columns and Collection Tubes are tested on a lot-to-lot basis by isolating genomic DNA from 50mg of young leaf samples. The purified DNA is quantified with a spectrophotometer and checked by electrophoresis.
Sample Size: Up to 100mg of fresh plant tissue, up to 25mg of dry plant tissue
Format: Spin column
Yield: 3 – 5 µg (from 100mg Arabdopsis thaliana leaf), 20 – 25 µg (from Nicotiana tabacum leaf)
Elution Volume: 30 – 300µl
Operation Time: within 30 minutes